产品信息
5' 测序引物及序列: pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC
3' 测序引物及序列: pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG
载体介绍
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
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- 基因合成
- 亚克隆构建
- 3,UTR验证实验
- 启动子合成及验证
- 慢病毒包装
- 腺病毒包装
- 稳转株构建
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