shRNA 慢病毒质粒构建及病毒包装
•选择靶点：TRC数据筛选目的基因靶点
https://portals.broadinstitute.org/gpp/public/gene/search_clones
示例：
Sequence Information
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Target Sequence:
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GCCAAGACCATCAGAAGTAAA
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Hairpin Sequence:
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5'-CCGG-GCCAAGACCATCAGAAGTAAA-CTCGAG-TTTACTTCTGATGGTCTTGGC-TTTTTG-3'
Oligo design for arrayed cloning:
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Forward sequence:
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5'-CCGGGCCAAGACCATCAGAAGTAAACTCGAGTTTACTTCTGATGGTCTTGGCTTTTTG-3'
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Reverse sequence:
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5'-AATTCAAAAAGCCAAGACCATCAGAAGTAAACTCGAGTTTACTTCTGATGGTCTTGGC-3'
Other clones with same target sequence:TRCN0000291961
数据库中可选human及mus的常规基因shRNA靶点，其他种属或lncRNA
请联系技术人员可提供免费设计服务！
该数据库是目前最为全面的shRNA慢病毒质粒文库，权阳生物拥有一致的序列PLKO.1 shRNA文库，可单独购买也可按照基因通路批量购买，价格低至200元/个克隆。
•选择载体：若PLKO.1 -puro载体不能满足您的实验需求，我们还有多种标记的载体可供选择
pLKO-U6-SH-PGK-copGFP-2A-PURO |
包装慢病毒后感染293A荧光图片（绿色荧光） |
pLKO-U6-SH-PGK-mCherry-2A-NEO |
包装慢病毒后感染293A荧光图片（红色荧光） |
还可选择其他载体标签比如用于体内成像的荧光素酶Luc等，订购产品后我们会提供详细的构建流程及shRNA序列的测序报告等COA文件及相关实验流程，免费提供对照质粒或病毒。
产品规格
•4+1 shRNA 慢病毒质粒套装（1500元）
4个目的基因的shRNA靶点+NC对照，每个质粒发货5ug及甘油菌
•即用型 4+1 shRNA 慢病毒质粒套装（2500元）
4个目的基因的shRNA靶点+NC对照，每个质粒发货50ug及甘油菌
•shRNA 慢病毒质粒指定序列构建（500元）
指定靶点的shRNA +NC对照，每个质粒发货5ug及甘油菌
•4+1 shRNA 慢病毒套装（已包装病毒）（10800元）
超离纯化的慢病毒，每个靶点提供1ml（滴度》1x10E8TU/ml）
•指定靶点的shRNA 慢病毒套装（已包装病毒）（2800元）
超离纯化的慢病毒，每个靶点提供1ml（滴度》1x10E8TU/ml）
•慢病毒包装质粒（1500元）
PsPAX2.0：1000ug（去内毒素）
PMD2.G ：500ug （去内毒素）
•慢病毒包装转染试剂（1200元）
规格为1ml/支，量大可优惠
相关文献引用
Y Wang, M Liu, Y Jin, S Jiang, J Pan - Cancer letters, 2017 - Elsevier
… Scramble (pLKO.1-puro-Non-target shRNA) or human HDAC1 specific target shRNA
(pLKO.1-puro-HDAC1-target shRNA) were from Transheep (Shanghai, China). Briefly, for
transfection of siRNA duplexes, shRNA constructs and overexpressing plasmids, nucleotides …
S Li, X Wu, Y Pei, W Wang, K Zheng, E Qiu… - DNA and cell …, 2019 - liebertpub.com
… Four
shRNAs targeting PTHR1 (shRNA_1: 5′-CCGGGC TACCAACTACTACTGGATTCTCG
AGAATCCAGTAG TAGTTGGTAGCTTTTT-3′; shRNA_2: 5′-CCGGGAGTC … GGAGTTTTT-3′)
and nontargeted control
shRNA were synthesized (
Transheep, Shanghai, China …
Y Li, R Liang, M Sun, Z Li, H Sheng, J Wang, P Xu… - Cancer letters, 2020 - Elsevier
… plasmids targeting PGM1, HDACs and AMPK were purchased from
Transheep Biological
Corporation. To generate knockdown cells, lentiviral
shRNA constructs were first transfected
along with … The targeting sequences of
shRNAs are detailed in supplementary information key …
Z Li, J Su, M Sun, J Song, H Sun, J Fan, G Chen… - Molecular and Cellular …, 2021 - Springer
… USA). Virus packaging and stable knockdown cell line generation. The
shRNA
plasmids targeting OCT1 were purchased from
Transheep Biological Corporation
(Shanghai, China). The
shRNA sequences were as follows: #1 …
R Ye, X Kuang, H Zeng, N Shao - 2020 - researchsquare.com
… The pSUPER- puro shRNA of TRIM3 and UBC9 were purchased from Transheep Bio. All clone
primer and siRNA obigonucleotides are listed as Supplemental Table 1. Estrogen Response
Element (ERE) was cloned into a pGL3 basic vector (Progema) …
